Hycult Biotech/TLR2，人，单克隆抗体TL2.1，功能研究抗体，低内毒素/HM2064-FS/0.5 mg
商品编号：
HM2064-FS
品牌：
hycultbiotech
市场价：
¥20832.00
美元价：
12499.20
产品分类：
标签抗体
公司分类：
Tag_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:TLR2,Human,mAbTL2.1,functionalstudyantibody,lowendotoxin
ThemonoclonalantibodyTL2.1recognizeshumanToll-likereceptor2(TLR2,CD282).Toll-likereceptors(TLR)arehighlyconservedthroughoutevolutionandareinvolvedintheinnatedefencetomanypathogens.InDrosophilatollisrequiredfortheanti-fungalresponse,whiletherelated18-wheelerisinvolvedinantibacterialdefences.Inmammals,TLRsareidentifiedastypeItransmembranesignalingreceptorswithpatternrecognitioncapABIlities.Theyhavebeenimplicatedintheinnatehostdefencetopathogens.TLR2isexpressedonmacrophages,smoothmuscle,lung,spleen,thymus,brainandADIposetissue.
TLR2hasbeenidentifiedasareceptorthatiscentraltotheinnateimmuneresponsetolipoproteinsofGram-negativebacteria,severalwholeGram-positivebacteria,aswellasareceptorforpeptidoglycanandlipoteichoicacidandotherbacterialcellmembraneproducts.AfunctionalinteractionbetweenTLR2andTLR6inthecellularresponsetovariousbacterialproductshasbeendiscovered.TLR2cooperateswithLY96tomediatetheinnateimmuneresponsetobacteriallipoproteinsandothermicrobialcellwallcomponents.ItcooperateswithTLR1tomediateteinnateimmuneresponsetobacteriallipoproteinsorlipopeptides.ItactsviaMYD88andTRAF6,leadingtoNF-κ-Bactivation,cytokinesecretionandtheinflammatoryresponse.TLR2alsopromotesapoptosisinresponsetolipoproteins.
Bacterialspeciesasdiverseasmycobacteria,spiRochetes,mycoplasma,S.aureus,BBurgdorferi,Tpallidum,MfermentansandStreptococcuspneumoniaehaveallbeenshowntomediatecellularactivationviaTLR2.
ThemonoclonalantibodyTL2.1isaTLR2functionblockingantibodythatisusefulforstudiesontheroleofTLR2asapatternrecognitionreceptorinmicrobialproductsinducedcytokineproductionbyTLR2bearingcellssuchashumanperipheralbloodmononuclearcells
TLR2hasbeenidentifiedasareceptorthatiscentraltotheinnateimmuneresponsetolipoproteinsofGram-negativebacteria,severalwholeGram-positivebacteria,aswellasareceptorforpeptidoglycanandlipoteichoicacidandotherbacterialcellmembraneproducts.AfunctionalinteractionbetweenTLR2andTLR6inthecellularresponsetovariousbacterialproductshasbeendiscovered.TLR2cooperateswithLY96tomediatetheinnateimmuneresponsetobacteriallipoproteinsandothermicrobialcellwallcomponents.ItcooperateswithTLR1tomediateteinnateimmuneresponsetobacteriallipoproteinsorlipopeptides.ItactsviaMYD88andTRAF6,leadingtoNF-κ-Bactivation,cytokinesecretionandtheinflammatoryresponse.TLR2alsopromotesapoptosisinresponsetolipoproteins.
Bacterialspeciesasdiverseasmycobacteria,spiRochetes,mycoplasma,S.aureus,BBurgdorferi,Tpallidum,MfermentansandStreptococcuspneumoniaehaveallbeenshowntomediatecellularactivationviaTLR2.
ThemonoclonalantibodyTL2.1isaTLR2functionblockingantibodythatisusefulforstudiesontheroleofTLR2asapatternrecognitionreceptorinmicrobialproductsinducedcytokineproductionbyTLR2bearingcellssuchashumanperipheralbloodmononuclearcells
Specifications:
| Catalognumber | HM2064-FS |
|---|---|
| Producttype | Monoclonalantibodies |
| Quantity | 0.5mg |
| Formulation | 0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel).Theendotoxinconcentrationis<24EU/mg,determinedwithHIT302LALAssay. |
| Application | Flowcytometry,Frozensections,Functionalstudies,Immunofluorescence,Immunoprecipitation,Paraffinsections,Westernblot |
| ApplicationNotes | FC:2 µgantibodyper300000cells;untreatedaswellas0.4%formaldehydefixedcellscanbeused;donotpermeabilizethecellsoruse4%faraformaldehydeasfixative!(Ref5) IF:HMECswerefixedwith4%paraformaldehydeinPBS,permeabilizedandblockedbeforestaining;antibodyconcentrationused5-10 µg/ml W:peripheralbloodmononuclearcells;non-reduced;1 µg/mlantibody;size~90kDa F:fixedinacetone;3%H2O2forblockadeofendogenousperoxidases;humantonsilsaspositivecontrol(useful,butcloneTL2.3(HM2066)ispreferred;Ref2) P:HOPE-fixed;alveolarepithelialcellstypeIIinhumanlungaspositivecontrol;1 µg/mlantibodyfor16hrat4 °C(Ref4) IP:lysedmonocyteswereimmunoprecipitatedwithantibody-conjugatedSepharose;size~90kDa(Ref2) FS:1 µg/5x107cells;antibodyblocksTNFarelease(inducedbybacteria)fromperipheralbloodmononuclearcells(Ref1) |
| Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
| Immunogen | RecombinanthumanTLR2producedbytheCHO/CD14reportercellline |
| Isotype | MouseIgG2a |
| Species | Human |
| Crossreactivity | Canine-Yes,Cynomolgusmonkey-Yes,Monkey-Yes,Rhesusmonkey-Yes |
| Alias | Toll-Likereceptor2,CD282,TLR2 Genename:TLR2 |
| PositiveControl | Peripheralbloodmononuclearcells,granulocytesandmonocytes. |
| References | 1.Lien,Eetal;Toll-likereceptor2functionsasapatternrecognitionreceptorfordiversebacterialproducts.JBiolChem1999,274:33419 2.Flo,Tetal;DifferentialexpressionofToll-likereceptor2inhumancells.JLeukocBiol2001,69:4743.Faure,Eetal;BacteriallipopolysaccharideandIFN-γinduceToll-likereceptor2andToll-likereceptor4expressioninhumanendothelialcells:roleofNF-κBactivation.JImmunol2001,166:2018 4.Droemann,Detal;Toll-likereceptor2isexpressedbyalveolarepithelialcellstypeIIandmacrophagesinthehumanlung.HistochemCellBiol2003,119:1035.Burgener,Ietal;AntibodiesspecificforhumanormurineToll-likereceptorsdetectcanineleukocytesbyflowcytometry.VetImmunolImmunopathol2008,124;184 6.Elsori,Detal;ProteinkinaseCisacriticalcomponentofDectin-1signalinginprimaryhumanmonocytes.JLeukocBiol2011,90:5997.Gaudreault,Eetal;TAK1contributestotheenhancedresponsivenessofLTB4-treatedneutrophilstoToll-likereceptorligands.Int.Immunol2012,24:693 8.Imamura,Yetal;Salivaryhistatin3inhibitsheatshockcognateprotein70-mediatedinflammatorycytokineproductionthroughtoll-likereceptorsinhumangingivalfibroblasts.JInflamm2014,11:4 |
| Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct. |
| Disease | Infectiousdiseases |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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