Hycult Biotech/BPI, Human, ELISA kit/HK314-02/2 x 96 det.
商品编号：
HK314-02
品牌：
hycultbiotech
市场价：
¥17320.00
美元价：
10392.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:BPI,Human,ELISAkit
TheantimicrobialproteinBPI(BactericidalPermeABIlityIncreasingprotein)isa55kDaproteinfoundintheprimary(azurophilic)granulesofhumanneutrophilsandhasalsobeendetectedonsurfaceofneutrophils,smallintestinalandoralepithelialcells.BPIisabactericidalcompoundthatispresentinpolymorphonuclearcells(PMN)andinlowerlevelsinthespecificgranulesofeosinophils.BPIpossesseshighaffinitytowardthelipidAregionoflipopolysaccharides(LPS)thatcomprisetheouterleafletofthegram-negativebacterialoutermembrane.BindingofBPItothelipidAmoietyofLPSexertsmultipleanti-infectiveactivitiesagainstgram-negativebacteria:1)cytotoxicityviasequentialdamagetobacterialouterandinnerlipidmembranes,2)neutralizationofgram-negativebacterialLPS,3)opsonizationofbacteriatoenhancephagocytosisbyneutrophils.AirwayepithelialcellsconstitutivelyexpresstheBPIgeneandproducetheBPIproteinand,therefore,BPImaybeacriticaldeterminantinthedevelopmentofLPS-triggeredairwaydisease.InflammationinducedbyLPSpossIBLycontributestothedevelopmentofrapidairflowdecline,aseriousandoftenfatalcomplicationofhematopoieticcelltransplantation.FurThermore,a21kDabioactiverecombinantfragmentofBPI,rBPI21,wasshowntoconferasurvivaladvantageagainstinvasivepneumococcaldiseasebybindingtothegram-positivebacterialpathogen,pneumolysin.InplasmaofhealthyindividualsBPIispresentatlevelsof<0.5 ng/ml,="" which="" increases="" approximately="" 10-fold="" during="" acute="" phase="" responses.="">
Specifications:
| Catalognumber | HK314-02 |
|---|---|
| Producttype | Assays |
| Quantity | 2x96det. |
| Standardrange | 100-25,000pg/ml |
| Detectionlevel | 250pg/ml |
| Workingvolume | 100µl/well |
| Species | Human |
| Application | ThehumanBPIELISAkitistobeusedfortheinvitroquantitativedeterminationofhumanBPIincellculturemedium,plasma,woundfluidandbronchoalveolarlavagefluid. |
| Disease | Infectiousdiseases |
| Principle | ThehumanBPIELISAisaready-to-usesolid-phaseenzyme-linkedimmunosorbentassaybasedonthesandwichprinciplewithaworkingtimeof4½hours.Theefficientformatof2plateswithtwelvedisposable8-wellstripsallowsfreechoiceofbatchsizefortheassay.Samplesandstandardsarecapturedbyasolidboundspecificantibody.BiotinylatedtracerantibodywillbindtocapturedhumanBPI.Streptavidin-peroxidaseconjugatewillbindtothebiotinylatedtracerantibody.Streptavidin-peroxidaseconjugatewillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofoxalicacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsofthehumanBPIstandards(log).ThehumanBPIconcentrationofsamples,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve. |
| Storageandstability | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultwiththeuseorderivationofthisproduct. |
| References | 1.Espinoza,Jetal;Antimicrobialpeptidesinamnioticfluid:defensins,calprotectinandbacterial/permeability-increasingproteininpatientswithmicrobialinvasionoftheamnioticcavity,intra-amnioticinflammation,pretermlaborandprematureruptureofmembranes.JMaternFetalNeonatalMed2003,13:2 2.Maris,Netal;Antiinflammatoryeffectsofsalmeterolafterinhalationoflipopolysaccharidebyhealthyvolunteers.AmJRespirCritCareMed2005,172:878 3.Gubern,Cetal;Naturalantibioticsandinsulinsensitivity,theroleofbactericidal/permeability-increasingprotein.Diabetes2006,55:216 4.Manco,Metal;EffectofmassiveweightlossoninflammatoryADIpocytokinesandtheinnateimmunesysteminmorbidlyobesewomen.JClinEndocrinolMetab2007,92:483 5.Strunk,Tetal;Reducedlevelsofantimicrobialproteinsandpeptidesinhumancordbloodplasma.ArchDisChildFetalNeonatalEd2009,94:F230 |
品牌介绍
荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。
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